The requirements for filtration can differ significantly between laboratory applications. Some samples need researchers to split material into size groups. Other samples have a mix of very small particles. If the goal is to take out the material first and then keep the small particles in a later filter stage using only one mesh size does not give enough control.
A good solution is to use multi‑stage filtering. Researchers can slowly separate material, by size using a series of mesh sizes.. In usual multi‑stage methods samples may have to be moved between many different filtration machines. These additional steps can make the process slower and increase sample handling.
The pluriStrainer® Maxi is designed to simplify this type of workflow. Its stackable design allows researchers to combine different mesh sizes into a single filtration setup. A Lab Strainer cascade allows a sample to move through several mesh sizes as part of one filtration setup. The pluriStrainer® Maxi is well suited to this type of arrangement, with 13 mesh sizes available from 5 µm to 2,000 µm. This range gives researchers more control when a sample contains particles that need to be separated at different size ranges.
Why Use Multiple Mesh Sizes?
A single filter is usually picked to keep particles that're within a certain size range. This method works fine when the sample is mostly uniform in size.. It can be less helpful when the sample has both large clusters and much smaller particles. For example if you pass a sample with clumps through a very fine mesh the large pieces may get stuck right on the surface. That can block the flow. Slow down the whole filtration process.
Using mesh sizes can be a better option. The sample can go through a sieve first. This keeps the bits while letting the smaller ones pass through. Then a finer sieve can catch the group of particles. Each step, in this process separates a part of the sample. Scientists take everything that stays on each sieve as one group. This way they can divide the sample into groups based on size. This approach is useful when different parts of the sample need to be collected or treated separately.
Understanding the pluriStrainer® Maxi
The pluriStrainer® Maxi is a large-volume sieving device designed for sample volumes ranging from more than 100 ml to more than 10 L. It is available in 13 mesh sizes:
5 µm
20 µm
40 µm
70 µm
100 µm
200 µm
300 µm
400 µm
500 µm
750 µm
1,000 µm
1,400 µm
2,000 µm
Laboratories can pick the mesh size that works best for the material they are working with and the amount of separation they need. The mesh sizes come in colors, which makes it easy for researchers to spot the right strainer fast during regular lab tasks. The pluriStrainer® Maxi has a GL45 thread so it fits perfectly with standard lab bottles. Adaptors are also available, for GL32 and GL80 bottles, giving options and better flexibility.
Building a Lab Strainer Cascade
The key feature that enables multi-stage filtration is the ability to stack pluriStrainer® Maxi units. Researchers can place strainers with different mesh sizes together to create a Lab Strainer cascade. The sample then passes through the different filtration stages in sequence.
The basic principle is simple. The first strainer can be chosen to keep particles while smaller pieces go through to the next stage. A finer mesh below can then catch particles. This setup creates size groups all in one system. For example researchers working with a sample that has clusters and tiny particles can start with a large mesh and then move to smaller mesh sizes one, after another. The exact mix depends on the sample and the size groups they need.
The advantage is that researchers don’t have to do one filtration move the material and then start a filtration with a different device. Multiple filtration steps can be connected into a setup.
Reducing Sample Transfers Between Filtration Steps
One of the challenges when using multi-stage filtration is moving the sample. In a process a scientist might filter a sample through one mesh take the liquid that comes through put it into a new container and then pass it through another mesh. Every time they move the sample it adds another step. The pluriStrainer® Maxi has a design that can be stacked. This means scientists can put mesh sizes in the order they need. The sample goes through each part as the filtering happens. Less moving can make the whole process simpler especially when dealing with amounts of sample. This is very important, for labs that do the kind of sample preparation many times and need to work quickly and efficiently.
Supporting Large-Volume Multi-Stage Filtration
Multi-stage filtration becomes more demanding as sample volume increases. Processing several liters through a series of small filtration devices can require repeated loading and collection. The pluriStrainer Maxi is made for bigger amounts of samples which means it works well when you need to handle more than 100 ml and up to more than 10 liters of material. The special high-flow channel system lets liquid move through and process more material. This way the filtration stays effective when dealing with large amounts.
The device has a tubing port that can be linked to a low-pressure system. This can make the filtration go quicker and let the samples be filled automatically. For labs that do filtration tasks these features can cut down on the need, for constant hands-on work.
Choosing Mesh Sizes for Different Stages
Selecting the right mesh sizes is central to successful multi-stage filtration. The appropriate combination depends on the material being processed and the size fractions researchers want to obtain. A larger mesh may be selected for the first stage when larger particles need to be retained or removed. Smaller mesh sizes can then be used in subsequent stages for progressively finer separation. The broad range offered by the pluriStrainer® Maxi gives laboratories considerable flexibility when designing these workflows.
Researchers can also adjust the mesh combination based on the application of being limited to a fixed filtration sequence. This flexibility helps laboratories that handle sample types or need to modify their separation approach as their research needs evolve.
Applications in Cell and Particle Separation
Multi-stage filtration can support applications where the size of particles, clusters or other material's important. The pluriStrainer® Maxi can be used for filtration of cell cultures, cell culture media, sera, additives, buffers and other liquid materials. The pluriStrainer® Maxi can also be used to enrich cell clusters or spheroids and split clusters into size fractions. In workflows involving cell separation technology size‑based filtration can provide a way to divide material before further processing.
For example researchers may use mesh sizes to separate larger cell clusters from smaller material. The resulting fractions can then be. Processed according to the needs of the experiment. This system can therefore support cell enrichment techniques where size is used as part of the sample preparation process.
Conclusion
Multi-stage filtration can be helpful when a sample has particles or cell material in different size ranges. Of putting all the sample through one mesh scientists can use different mesh sizes in different steps to split the material into separate parts.
The pluriStrainer® Maxi is made to support this kind of system. It can be. Comes in 13 mesh sizes from 5 µm to 2,000 µm. By using units with mesh sizes scientists can build a Lab Strainer cascade and move the sample through many filtration steps without having to move it manually from one device to another each time. Features like its big volume capacity, fast flow channel system, bottle compatibility and optional low-pressure and automated refilling options can also help when dealing with amounts of filtration.
This system can be used for lab tasks such, as cell cultures, cell clusters, spheroids, environmental samples and other liquids that contain particles. Scientists can choose the mesh size for each step according to the material they are working with and the separation they require. This allows them to set up a multi‑stage filtration process that matches the requirements of a task.

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